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Fr«ßƒ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ²<?xml version="1.0"?><Features nextValidID="8"><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5645-5749" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5575" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4784-5575" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5576-5644" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2643-3242" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4025-4613" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Kozak sequence" type="regulatory" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="794-803" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="5" name="EF-1Î± promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="829-2010" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong constitutive promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="bGH poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="324-548" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="Î²-globin poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3407-3462" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;rabbit&amp;nbsp;Î²-globin polyadenylation signal (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3568131&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3568131&quot;&gt;Gil and Proudfoot, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  ª<?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="5406-5425" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5406-5425" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="5406-5425" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5406-5425" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="1" name="BGH-rev" sequence="TAGAAGGCACAGTCGAGG" description="Bovine growth hormone terminator, reverse primer. Also called BGH reverse"><BindingSite location="536-553" boundStrand="0" annealedBases="TAGAAGGCACAGTCGAGG" meltingTemperature="55"><Component hybridizedRange="536-553" bases="TAGAAGGCACAGTCGAGG"/></BindingSite><BindingSite simplified="1" location="536-553" boundStrand="0" annealedBases="TAGAAGGCACAGTCGAGG" meltingTemperature="55"><Component hybridizedRange="536-553" bases="TAGAAGGCACAGTCGAGG"/></BindingSite></Primer><Primer recentID="2" name="Bglob-pA-R" sequence="TTTTGGCAGAGGGAAAAAGA" description="Rabbit beta-globin polyA region, reverse primer"><BindingSite location="3341-3360" boundStrand="1" annealedBases="TTTTGGCAGAGGGAAAAAGA" meltingTemperature="54"><Component hybridizedRange="3341-3360" bases="TTTTGGCAGAGGGAAAAAGA"/></BindingSite><BindingSite simplified="1" location="3341-3360" boundStrand="1" annealedBases="TTTTGGCAGAGGGAAAAAGA" meltingTemperature="54"><Component hybridizedRange="3341-3360" bases="TTTTGGCAGAGGGAAAAAGA"/></BindingSite></Primer><Primer recentID="3" name="EF1a-F" sequence="TCAAGCCTCAGACAGTGGTTC" description="Human elongation factor-1a promoter, forward primer"><BindingSite location="860-880" boundStrand="1" annealedBases="TCAAGCCTCAGACAGTGGTTC" meltingTemperature="58"><Component hybridizedRange="860-880" bases="TCAAGCCTCAGACAGTGGTTC"/></BindingSite><BindingSite simplified="1" location="860-880" boundStrand="1" annealedBases="TCAAGCCTCAGACAGTGGTTC" meltingTemperature="58"><Component hybridizedRange="860-880" bases="TCAAGCCTCAGACAGTGGTTC"/></BindingSite></Primer><Primer recentID="4" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="3853-3870" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="3853-3870" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="3853-3870" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="3853-3870" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="5" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="4104-4123" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4104-4123" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="4104-4123" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4104-4123" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="6" name="Puro-F" sequence="GCAACCTCCCCTTCTACGAGC" description="Puromycin resistance gene, forward primer"><BindingSite location="3138-3158" boundStrand="0" annealedBases="GCAACCTCCCCTTCTACGAGC" meltingTemperature="60"><Component hybridizedRange="3138-3158" bases="GCAACCTCCCCTTCTACGAGC"/></BindingSite><BindingSite simplified="1" location="3138-3158" boundStrand="0" annealedBases="GCAACCTCCCCTTCTACGAGC" meltingTemperature="60"><Component hybridizedRange="3138-3158" bases="GCAACCTCCCCTTCTACGAGC"/></BindingSite></Primer><Primer recentID="7" name="Puro-R" sequence="GTGGGCTTGTACTCGGTCAT" description="Puromycin resistance gene, reverse primer. Also called puro-variant-R"><BindingSite location="2642-2661" boundStrand="1" annealedBases="GTGGGCTTGTACTCGGTCAT" meltingTemperature="59"><Component hybridizedRange="2642-2661" bases="GTGGGCTTGTACTCGGTCAT"/></BindingSite><BindingSite simplified="1" location="2642-2661" boundStrand="1" annealedBases="GTGGGCTTGTACTCGGTCAT" meltingTemperature="59"><Component hybridizedRange="2642-2661" bases="GTGGGCTTGTACTCGGTCAT"/></BindingSite></Primer><Primer recentID="8" name="rbglobpA-R" sequence="CCCATATGTCCTTCCGAGTG" description="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R"><BindingSite location="3460-3479" boundStrand="1" annealedBases="CCCATATGTCCTTCCGAGTG" meltingTemperature="57"><Component hybridizedRange="3460-3479" bases="CCCATATGTCCTTCCGAGTG"/></BindingSite><BindingSite simplified="1" location="3460-3479" boundStrand="1" annealedBases="CCCATATGTCCTTCCGAGTG" meltingTemperature="57"><Component hybridizedRange="3460-3479" bases="CCCATATGTCCTTCCGAGTG"/></BindingSite></Primer></Primers>
  °<Notes>
<UUID>82fad3ce-5852-4f75-a66c-1f058468b8d0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pSBbi-Pur</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene.</Description>
<Created UTC="20:39:56">2021.5.12</Created>
<LastModified UTC="20:39:56">2021.5.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference journalName="Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821." authors="Kowarz E, Loescher D, Marschalek R" pubMedID="25650551" journal="Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821." title="Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines." type="Journal Article"/>
</References>
</Notes>
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